custom made spotted microarray Search Results


custom cdna spotted microarray  (Wageningen University and Research)
90
Wageningen University and Research custom cdna spotted microarray
Custom Cdna Spotted Microarray, supplied by Wageningen University and Research, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/custom cdna spotted microarray/product/Wageningen University and Research
Average 90 stars, based on 1 article reviews
custom cdna spotted microarray - by Bioz Stars, 2026-06
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customized printheads silicon microarray spotting pins  (Parallel Synthesis Technologies)
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Parallel Synthesis Technologies customized printheads silicon microarray spotting pins
Customized Printheads Silicon Microarray Spotting Pins, supplied by Parallel Synthesis Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
customized printheads silicon microarray spotting pins - by Bioz Stars, 2026-06
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custom cdna spotted microarray  (CSIRO Livestock)
90
CSIRO Livestock custom cdna spotted microarray
Custom Cdna Spotted Microarray, supplied by CSIRO Livestock, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/custom cdna spotted microarray/product/CSIRO Livestock
Average 90 stars, based on 1 article reviews
custom cdna spotted microarray - by Bioz Stars, 2026-06
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custom made spotted microarray  (Full Moon BioSystems)
90
Full Moon BioSystems custom made spotted microarray
Specificity and sensitivity of used anti-methylcytosine antibody. ( A ) The antibody properties were assessed by an indirect immunofluorescence assay, in which the monoclonal anti-MeC antibody for this study — used at the concentration of 1 μg/ml in combination with a secondary antibody (Cy3-conjugated anti-mouse IgG1 at 5 μg/ml), i.e. at the same concentrations as in the cellular assay — was hybridized to a spotted array with two types of short 24-mer oligonucleotides immobilized onto a glass slide: C-oligo that included two CG dinucleotides and its methylated counterpart, the MeC-oligo printed at various dilutions that correlate with different approximate CpG copy numbers (10 10 –10 4 ) . Each DNA probe was spotted as octuple. The specific antibody, detected with a <t>microarray</t> scanner at 5 microns resolution, shows best signal-to-noise (background and non-specific binding to unmethylated C-oligo) ratio at a copy number of 10 10 . The signal (false-colored in green) decreases in a CpG copy number-dependent manner. ( B ) Similar average intensities were obtained, when a sub-area (magenta box in Figure A) of the same array was subjected to confocal scanning microscopy at 200 nm horizontal resolution. The line scan (magenta) shows the more detailed intensity profile across the four different types of spots and the intermediate gaps (coated glass slide/background).
Custom Made Spotted Microarray, supplied by Full Moon BioSystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/custom made spotted microarray/product/Full Moon BioSystems
Average 90 stars, based on 1 article reviews
custom made spotted microarray - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier

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Specificity and sensitivity of used anti-methylcytosine antibody. ( A ) The antibody properties were assessed by an indirect immunofluorescence assay, in which the monoclonal anti-MeC antibody for this study — used at the concentration of 1 μg/ml in combination with a secondary antibody (Cy3-conjugated anti-mouse IgG1 at 5 μg/ml), i.e. at the same concentrations as in the cellular assay — was hybridized to a spotted array with two types of short 24-mer oligonucleotides immobilized onto a glass slide: C-oligo that included two CG dinucleotides and its methylated counterpart, the MeC-oligo printed at various dilutions that correlate with different approximate CpG copy numbers (10 10 –10 4 ) . Each DNA probe was spotted as octuple. The specific antibody, detected with a microarray scanner at 5 microns resolution, shows best signal-to-noise (background and non-specific binding to unmethylated C-oligo) ratio at a copy number of 10 10 . The signal (false-colored in green) decreases in a CpG copy number-dependent manner. ( B ) Similar average intensities were obtained, when a sub-area (magenta box in Figure A) of the same array was subjected to confocal scanning microscopy at 200 nm horizontal resolution. The line scan (magenta) shows the more detailed intensity profile across the four different types of spots and the intermediate gaps (coated glass slide/background).

Journal: BMC Pharmacology & Toxicology

Article Title: 3-D DNA methylation phenotypes correlate with cytotoxicity levels in prostate and liver cancer cell models

doi: 10.1186/2050-6511-14-11

Figure Lengend Snippet: Specificity and sensitivity of used anti-methylcytosine antibody. ( A ) The antibody properties were assessed by an indirect immunofluorescence assay, in which the monoclonal anti-MeC antibody for this study — used at the concentration of 1 μg/ml in combination with a secondary antibody (Cy3-conjugated anti-mouse IgG1 at 5 μg/ml), i.e. at the same concentrations as in the cellular assay — was hybridized to a spotted array with two types of short 24-mer oligonucleotides immobilized onto a glass slide: C-oligo that included two CG dinucleotides and its methylated counterpart, the MeC-oligo printed at various dilutions that correlate with different approximate CpG copy numbers (10 10 –10 4 ) . Each DNA probe was spotted as octuple. The specific antibody, detected with a microarray scanner at 5 microns resolution, shows best signal-to-noise (background and non-specific binding to unmethylated C-oligo) ratio at a copy number of 10 10 . The signal (false-colored in green) decreases in a CpG copy number-dependent manner. ( B ) Similar average intensities were obtained, when a sub-area (magenta box in Figure A) of the same array was subjected to confocal scanning microscopy at 200 nm horizontal resolution. The line scan (magenta) shows the more detailed intensity profile across the four different types of spots and the intermediate gaps (coated glass slide/background).

Article Snippet: Antibody testing was performed by an immunofluorescence assay utilizing a custom made spotted microarray (Full Moon Biosystems) comprising multiple copies of two synthesized 24-mer oligonucleotide probes that were immobilized onto glass microscopic slides: 5 ′ -TCGTTTTTTTTTTTTTTTTTTCGT-3 ′ (C-oligo) (MWG Biotech), and its counterpart 5 ′ -T me CGTTTTTTTTTTTTTTTTTT me CGT-3 ′ (MeC-oligo) (Biopolymers-Thermo Scientific), in which the two cytosine molecules were replaced by methylcytosine.

Techniques: Immunofluorescence, Concentration Assay, Methylation, Microarray, Binding Assay, Microscopy